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Tissue Engineering of a Human 3D in vitro Tumor Test System
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Making In Vitro Tumor Models Whole Again.

Kenny Zhuoran Wu1, Christabella Adine1, Aleksandr Mitriashkin1

  • 1Department of Biomedical Engineering, College of Design and Engineering, National University of Singapore, Singapore, 119276, Singapore.

Advanced Healthcare Materials
|January 31, 2023
PubMed
Summary

Patient-derived tumor explant (PDE) cultures retain the tumor microenvironment (TME) for personalized drug testing. Technologies to improve PDE culture viability and throughput are crucial for advancing cancer precision medicine.

Keywords:
drug screeningin vitro tumor modelspatient-derived tumor explantspersonalized oncologytumor microenvironments

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Area of Science:

  • Oncology
  • Biomedical Engineering
  • Translational Medicine

Background:

  • Patient-derived in vitro tumor models lack crucial tumor microenvironment (TME) components, limiting personalized drug testing.
  • In vivo models are expensive, slow, and not high-throughput, hindering drug development.
  • Ex vivo patient-derived tumor explant (PDE) cultures preserve the intact TME but face challenges in viability and scalability.

Purpose of the Study:

  • To review limitations of current patient-derived tumor explant (PDE) cultures.
  • To discuss technologies for overcoming PDE culture challenges.
  • To highlight the potential of engineered PDE cultures for personalized cancer medicine.

Main Methods:

  • Review of existing literature on patient-derived tumor explant (PDE) cultures.
  • Analysis of limitations including low-throughput and poor long-term viability.
  • Exploration of technological advancements for enhancing PDE cultures.

Main Results:

  • Patient-derived tumor explant (PDE) cultures offer a holistic approach to studying the tumor microenvironment (TME).
  • Key limitations of PDE cultures include scalability and maintaining long-term viability.
  • Emerging technologies show promise in addressing these limitations.

Conclusions:

  • Engineered patient-derived tumor explant (PDE) cultures can overcome current limitations.
  • These advanced cultures are essential for accurate drug testing and personalized medicine in oncology.
  • Resurrecting and optimizing PDE cultures is vital for immune-oncology and precision cancer treatment.