Draft Genome Assemblies of Phage AP50c-Resistant Derivatives of Bacillus anthracis Sterne Strain 7702 Lacking Plasmid...

Area of Science:

• Microbiology and Genomics
• Bacterial Genetics
• Molecular Biology

Background:

• The attenuated Bacillus anthracis Sterne strain 7702 is a widely studied model organism.
• Bacterial viruses (bacteriophages) are crucial tools for studying bacterial genetics and can be used to select for specific mutations.
• The S-layer is a surface protein layer found in many bacteria, contributing to cell shape, adhesion, and protection.

Purpose of the Study:

• To perform draft genome sequencing of the parent Bacillus anthracis Sterne strain 7702.
• To sequence the genomes of several derivatives of strain 7702 that exhibit resistance to the bacteriophage AP50c.
• To investigate mutations within genes involved in the synthesis and assembly of the bacterial S-layer in phage-resistant mutants.

Main Methods:

• Whole-genome sequencing of multiple Bacillus anthracis strains.
• Bioinformatic analysis of draft genome assemblies.
• Comparative genomics to identify genetic differences between parent and mutant strains.

Main Results:

• Draft genome assemblies were successfully generated for the parent strain and phage-resistant mutants.
• Genetic variations were identified in the phage-resistant derivatives compared to the parent strain.
• Mutations were characterized in genes associated with the S-layer pathway in selected mutants.

Conclusions:

• The study provides valuable genomic data for Bacillus anthracis Sterne strain 7702 and its phage-resistant mutants.
• The findings offer insights into the genetic mechanisms underlying bacteriophage resistance in Bacillus anthracis.
• This research contributes to understanding the genetic regulation of S-layer formation and its potential role in phage resistance.