Related Experiment Video
Updated: Aug 12, 2025

Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface
Published on: February 21, 2011
The impact of puff frequency on respirable particulate matter in mainstream cigarette smoke
Alfayo K Maiyo1, Benjamin K Korir2, Joshua K Kibet2
1Department of Chemistry and Biochemistry, Moi University, Eldoret, Kenya.
Background:
Inhalation of particulate matter (PM) from cigarette smoke is hazardous to smokers and non-smokers. This contribution simulates the deposition of cigarette PM on the lung surface by trapping tobacco smoke particulates on Croton megalocarpus biochar. This study investigated one commercial cigarette (MM) and one local cigarette (RR).
Methodology:
Biochar was incorporated into the filters of MM and RR cigarettes in order to adsorb PM from mainstream cigarette smoke. A weighed 5 mg of biochar with adsorbed cigarette PM was analyzed using a scanning electron microscope and a Fourier transform infrared spectrometer. The size distribution of cigarette smoke particulates was processed using ImageJ software.
Results:
At 15 s puff time, the mean particulate diameters for the commercial and the local cigarettes, respectively, can be classified as coarse ≈ PM10 . Conversely, the mean particulate diameter at 2 s puff time for the commercial cigarette falls under the ultrafine classification of ≤PM2.5 , whereas at the same puff time, the mean particulate diameter for the local cigarette was approximately PM2.5 . Data from Fourier transform infrared spectroscopy indicate the PM in the two model cigarettes contains aromatic structures that feature the C=C bond characterized by an intense absorption band at δs (1600 cm-1 ).
Conclusions:
This study found that PM in mainstream cigarette smoke depends on puff time. Although cigarette smoking was conducted for two model cigarettes, this study can be extended to any other form of cigarette. Moreover, this study emphasizes the need for comprehensive studies on real-world cigarette smoking conditions, taking into account cigarette smokers who use larger puff volumes.
More Related Videos
08:39Generation of Electronic Cigarette Aerosol by a Third-Generation Machine-Vaping Device: Application to Toxicological Studies
Published on: August 25, 2018
09:50Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke
Published on: February 12, 2015
Related Concept Videos
Factors Affecting Pulmonary Ventilation
Alveolar Surface Tension
The alveolar fluid lines the luminal surface of the alveoli and exerts a force called surface tension. This force is caused by the polar water molecules in the liquid being more strongly attracted to each...
Chronic Obstructive Pulmonary Disease-II: Pathophysiology
Chronic Inflammation
Stimulants
Cocaine can be administered via snorting, injection, or smoking. It primarily functions by blocking the reuptake of dopamine, resulting in a euphoric high characterized by an intense sensation of happiness and...
Chronic Obstructive Pulmonary Disease-I: Introduction
Chronic Obstructive Pulmonary Disease
Smoking is a primary risk factor for COPD, with over 80% of patients having a history of it. Patients typically experience progressive dyspnea or labored breathing, frequent coughing, and recurrent pulmonary infections. Many eventually succumb to respiratory failure, characterized by...
Factors Affecting Respiration