Related Experiment Video
Updated: Aug 12, 2025

06:06
Adjunctive Diode Laser Therapy and Probiotic Lactobacillus Therapy in the Treatment of Periodontitis and Peri-Implant Disease
Published on: May 9, 2022
2.6K
Intracanal Microbiological Contamination Reduction Using Low-intensity Laser Associated with a Photosensitizer - In
Marco Antonio Leon-Roman1, Juliana Kowalesky2, Fernanda M Lopes Kubitza2
1Surgery Department, USP FMVZ, Sao Paulo, SP, Brazil.
Journal of Veterinary Dentistry
|February 1, 2023
Summary
Photodynamic therapy with azulene and low-intensity laser effectively eliminates bacteria and yeast in canine root canals, offering a viable alternative to standard treatments.
Area of Science:
- Veterinary Dentistry
- Endodontics
- Photodynamic Therapy
Background:
- Dental infections in animals require effective disinfection methods.
- Standard root canal preparation has limitations in complete microbial elimination.
Purpose of the Study:
- To evaluate the efficacy of photodynamic therapy (PDT) using azulene and low-intensity laser in canine endodontic treatment.
- To compare PDT with standard chemical-surgical preparation for intracanal disinfection.
Main Methods:
- Twenty dogs with single-rooted teeth underwent endodontic treatment.
- Two groups were formed: standard preparation followed by PDT, and PDT followed by standard preparation.
- Photodynamic therapy utilized azulene as a photosensitizer and a low-intensity laser.
Main Results:
- Intracanal PDT demonstrated efficiency in reducing bacterial and Enterococcus loads.
- PDT also reduced yeast contamination within the root canals.
- PDT showed comparable efficacy to standard chemical-surgical preparation.
- The order of applying PDT and standard preparation did not affect disinfection outcomes.
Conclusions:
- Photodynamic therapy with azulene and low-intensity laser is an effective intracanal disinfection method.
- PDT is a feasible alternative for veterinary dentistry, potentially improving treatment outcomes.
- The application sequence of PDT does not influence its effectiveness in disinfection.

