Visible light responsive photoacids for subcellular pH and temperature correlated fluorescence sensing
Yu Cheng1,2, Xueqing Ma1,2, Jingying Zhai3,4
1Department of Chemistry, Southern University of Science and Technology, Shenzhen, 518055, China. xiexj@sustech.edu.cn.
Chemical Communications (Cambridge, England)
|February 1, 2023
Summary
Liao
Area of Science:
- Biochemistry and Molecular Biology
- Chemical Biology
- Cell Biology
Background:
- Photoacids (PAs) are established visible light-responsive photoswitches.
- Their temperature-dependent thermal relaxation properties are well-documented.
Purpose of the Study:
- To introduce photoacids as novel fluorescent temperature sensors within cellular environments.
- To explore the dual-sensing capabilities of photoacids for both temperature and pH.
Main Methods:
- Utilizing the temperature-dependent thermal relaxation of photoacids from ring-closed to ring-opened forms.
- Measuring the logarithmic lifetime (ln τ) of the ring-closed spiro-form.
- Observing the fluorescent response of ring-opened PAs in relation to lysosomal pH.
Main Results:
- Demonstrated an excellent linear correlation between logarithmic lifetime and the reciprocal of temperature.
- Confirmed the ability of fluorescent ring-opened PAs to visualize lysosomes.
- Showcased the responsiveness of PAs to changes in lysosomal pH.
Conclusions:
- Photoacids serve as effective fluorescent probes for intracellular temperature sensing.
- Photoacids exhibit potential for simultaneous sensing of subcellular pH and temperature.
- This research opens new avenues for advanced cellular imaging and diagnostics.
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