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Related Experiment Video

Updated: Aug 12, 2025

Synthesis of Monocyte-targeting Peptide Amphiphile Micelles for Imaging of Atherosclerosis

Synthesis of Monocyte-targeting Peptide Amphiphile Micelles for Imaging of Atherosclerosis

Published on: November 17, 2017

MiR-146a encapsulated liposomes reduce vascular inflammatory responses through decrease of ICAM-1 expression,

Donald Ho¹, Tyler O Lynd², Claire Jun³

¹Department of Pediatric Dentistry, University of Alabama at Birmingham, AL, 35294, USA.

|February 1, 2023

Summary

Liposomes delivering microRNA 146a (miR-146a) effectively reduced vascular inflammation in cells. This targeted approach shows promise for treating inflammatory vascular diseases like atherosclerosis.

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Area of Science:

Biomedical Engineering
Molecular Biology
Cardiovascular Research

Background:

Vascular insults trigger inflammatory responses involving endothelial cells, smooth muscle cells, and macrophages, contributing to atherosclerosis.
MicroRNA 146a (miR-146a) exhibits anti-inflammatory properties by regulating the nuclear factor kappa beta (NF-κβ) pathway.

Purpose of the Study:

To investigate the therapeutic potential of exogenous miR-146a encapsulated in liposomes for mitigating vascular inflammation.
To assess the efficacy of miR-146a liposomes in reducing inflammatory markers and cellular responses in stimulated vascular cells and macrophages.

Main Methods:

Liposomes were engineered for uniform size, stability, and high encapsulation efficiency of miR-146a.
Transfection efficiency of miR-146a liposomes in human aortic endothelial cells (HAECs), smooth muscle cells (SMCs), and U937 monocytes was confirmed.
Inflammatory responses, including ICAM-1 expression, monocyte adhesion, pro-inflammatory cytokine production (TNF-α, IL-1β), oxidized low-density lipoprotein (ox-LDL) uptake, and foam cell formation were measured.

Main Results:

miR-146a liposomes demonstrated successful transfection and were stable.
Treatment reduced vascular inflammation in HAECs and SMCs by inhibiting ICAM-1 and decreasing monocyte adhesion.
In macrophages, miR-146a liposomes suppressed pro-inflammatory cytokines, reduced ox-LDL uptake, and inhibited foam cell formation.

Conclusions:

Exogenous miR-146a encapsulated in liposomes effectively reduces multiple inflammatory mediators in vascular cells and macrophages.
miR-146a liposomes represent a promising therapeutic strategy for managing inflammatory vascular diseases, including atherosclerosis.