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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

113
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Updated: Aug 12, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
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Recent progress in nucleic acid detection with CRISPR.

Frank X Liu1, Johnson Q Cui1, Zhihao Wu2

  • 1Department of Mechanical and Aerospace Engineering, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong. meshyao@ust.hk.

Lab on a Chip
|February 1, 2023
PubMed
Summary
This summary is machine-generated.

CRISPR technology is revolutionizing molecular diagnostics due to its programmable specificity. This review explores CRISPR-based diagnostic systems, detailing amplification strategies and future biosensing prospects.

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Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Bioengineering

Background:

  • CRISPR-based biotechnologies offer advanced capabilities for molecular diagnostic systems.
  • The programmability and high specificity of CRISPR are key attributes for its broad utility.

Purpose of the Study:

  • To review the principles of Class 2 CRISPR nucleases in molecular diagnostics.
  • To present CRISPR-based diagnostic schemes, including amplification-assisted and amplification-free strategies.
  • To discuss the integration of bioengineering methodologies with CRISPR for diagnostics.

Main Methods:

  • Illustrating the principles of Class 2 CRISPR nucleases.
  • Presenting CRISPR-based diagnostic schemes.
  • Highlighting recent advances and bioengineering integration.

Main Results:

  • CRISPR nucleases, derived from immune systems, are effective for molecular diagnostics.
  • Both amplification-assisted and amplification-free strategies are viable for CRISPR-based diagnostics.
  • Bioengineering principles can be integrated with CRISPR for enhanced biosensing.

Conclusions:

  • CRISPR-mediated nucleic acid detection is a rapidly advancing field.
  • Understanding CRISPR nucleases and design concepts is crucial for diagnostic applications.
  • Future developments hold exciting prospects for CRISPR-based biosensing.