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A portable electrochemical sensing platform for serotonin detection based on surface-modified carbon fiber
Jinjing Han1,2,3, Justin M Stine1,2,3, Ashley A Chapin2,3,4
1Department of Electrical and Computer Engineering, University of Maryland, College Park, MD 20742, USA,. ghodssi@umd.edu.
Analytical Methods : Advancing Methods and Applications
|February 1, 2023
Summary
This study presents a novel, low-cost electrochemical sensor for detecting serotonin (5-HT) in biological fluids. The portable system demonstrates high sensitivity and selectivity, enabling potential point-of-care diagnostics for various health conditions.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Neuroscience
Background:
- Serotonin (5-HT) is a crucial neurotransmitter regulating physiological functions.
- Imbalances in serotonin homeostasis are linked to neurodegenerative disorders, depression, and serotonin syndrome.
- Accurate detection of serotonin in biological fluids is vital for early disease diagnosis.
Purpose of the Study:
- To develop a novel, simple, and low-cost electrochemical sensing platform for serotonin detection.
- To enhance sensitivity and selectivity for serotonin detection in artificial biological fluids.
- To validate the feasibility of the developed system for point-of-care analysis.
Main Methods:
- Fabrication of a portable electrochemical workstation with customized electrodes.
- Modification of carbon fiber microelectrodes with Nafion/carbon nanotubes (Nafion-CNT/EC CFMEs).
- Integration of Ag/AgCl reference and Pt counter electrodes for a complete sensing system.
Main Results:
- The Nafion-CNT/EC CFMEs exhibited improved sensitivity and selectivity for serotonin.
- The portable sensing platform achieved a sensitivity of 0.074 μA μM⁻¹ and a limit of detection (LOD) of 140 nM.
- The system demonstrated high recovery rates when analyzing serotonin spiked in artificial urine samples.
Conclusions:
- The developed portable electrochemical sensing platform offers outstanding performance for serotonin detection.
- The system confirms the feasibility of using electrochemical methods for point-of-care serotonin analysis.
- This technology holds potential for early diagnosis and monitoring of serotonin-related health conditions.
