Prevalence and clonal diversity of methicillin-resistant Staphylococcus aureus and methicillin-resistant

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Seyda Cengiz1, Sitkican Okur2, Cihan Oz1

  • 11Department of Microbiology, Faculty of Veterinary Medicine, Ataturk University, 25240, Erzurum, Turkey.

Acta Microbiologica Et Immunologica Hungarica
|February 1, 2023
PubMed
Abstract

Insights

Methicillin-resistant Staphylococcus strains were identified in dogs and cats, with some strains showing genetic links between animals and humans. This highlights the need for collaborative One Health strategies to control their spread.

Area of Science:

  • Veterinary Microbiology
  • Antimicrobial Resistance
  • Molecular Epidemiology

Background:

  • Methicillin-resistant Staphylococcus (MRS) poses a significant threat in both human and animal health.
  • Understanding the prevalence and genetic characteristics of MRS in companion animals is crucial for public health.

Purpose of the Study:

  • To determine the prevalence, genetic diversity, and antimicrobial resistance patterns of Staphylococcus aureus and Staphylococcus pseudintermedius from dogs and cats.
  • To investigate potential epidemiological links between animal and human strains.

Main Methods:

  • Isolation and identification of coagulase-positive staphylococci from canine and feline eye discharges.
  • Phenotypic antimicrobial susceptibility testing and detection of the mecA gene via PCR.
  • Phylogenetic analysis using repetitive sequence-based PCR (rep-PCR) and multi-locus sequence typing (MLST).

Main Results:

  • 12 coagulase-positive staphylococci were identified (6 S. aureus, 6 S. pseudintermedius).
  • Phenotypic methicillin resistance was observed in 50% of S. aureus and 16.7% of S. pseudintermedius.
  • MLST revealed epidemiologically related strains (ST241, ST2361, ST4155, ST7217), with some linked to previously reported cases.

Conclusions:

  • Epidemiologically related Staphylococcus strains are transferable between animals and humans.
  • Collaborative strategies between human and veterinary medicine are essential under the One Health concept to combat MRS.
Keywords:
catsdogsmethicillin-resistant S. aureusmethicillin-resistant S. pseudintermedius

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