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Related Concept Videos

High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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Anticoagulant Drugs: Low-Molecular-Weight Heparins01:30

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Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
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High-Performance Liquid Chromatography: Instrumentation00:57

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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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Types Of Column Chromatography01:29

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The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
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Principles Of Column Chromatography01:13

Principles Of Column Chromatography

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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Affinity Chromatography

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Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Related Experiment Video

Updated: Aug 12, 2025

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
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[Advances in heparin structural analysis by chromatography technologies].

Yilan Ouyang1, Lin Yi1, Luyun Qiu1

  • 1College of Pharmaceutical Sciences，Suzhou Medical College of Soochow University，Suzhou 215127，China.

Se Pu = Chinese Journal of Chromatography
|February 1, 2023
PubMed
Summary
This summary is machine-generated.

Heparin (Hp) and its derivatives are complex drugs requiring advanced analysis. This review details chromatographic methods for structural elucidation and quality control of Hp drugs, crucial for safety and production.

Keywords:
chromatographydisaccharide composition analysisheparin （Hp）low molecular weight heparin （LMWHs）monosaccharide composition analysisoligosaccharide analysispolysaccharide analysisreview

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Pharmacology

Background:

  • Heparin (Hp) is a vital anticoagulant drug derived from porcine intestinal mucosa, with significant global market value.
  • Low-molecular-weight heparins (LMWHs) offer improved clinical profiles over unfractionated Hp but share structural complexities.
  • The inherent heterogeneity of Hp and LMWHs poses significant challenges for structural analysis, quality control, and production optimization.

Approach:

  • This review systematically organizes and analyzes recent advancements in chromatographic methods for Hp drug analysis.
  • It covers the identification and composition analysis of monosaccharides, disaccharides, oligosaccharides, and polysaccharides within Hp.
  • The paper evaluates the applications, innovations, and limitations of various chromatographic techniques employed in Hp research.

Key Points:

  • Chromatographic methods are the most extensively studied and utilized for Hp structural analysis and quality control.
  • Accurate structural elucidation of Hp drugs is critical for ensuring clinical safety, optimizing manufacturing, and exploring new applications.
  • This work provides a comprehensive overview of chromatographic applications, aiding researchers and industry professionals.

Conclusions:

  • The review highlights the indispensable role of chromatography in understanding the complex structures of Heparin drugs.
  • It serves as a valuable reference for structural analysis and quality control, guiding future research and development.
  • The insights presented aim to foster advancements in Heparin drug characterization and therapeutic applications.