Diagnosing Different Types of Bacteria on Culex Mosquito Spp. (Diptera: Culicidae) in Baghdad

Introduction The genus of mosquitoes is also one of the important factors affecting the diversity of bacteria; the aim of the current research is to isolate the types of bacteria from adult mosquitoes that grow near stagnant water ponds in Baghdad Governorate, in order to collect information about bacterial species common in mosquitoes present in the area. Methods A number of Culicidae mosquito specimens were collected from Abu Ghraib, Baghdad, and the isolation of microorganisms from mosquitoes after prepared culture media and then a biochemical study of the identification characteristics of bacterial isolates was done. Result Some morphological traits were studied, and their production of catalase and oxidase enzymes was determined; 2×10³ bacterial cells/ml of different genera and species were isolated on a solid agar nutrient medium for insect models. Bacterial colonies appeared with shapes ranging from pinworms to colonies with flat edges, white color, and smooth surfaces, and some types of fungi were isolated with a number of 1×10² cells/ml. Bacterial cells were isolated on the optional MacConkey medium, only less than 100 cells/ml when using the direct method, and the number reached 1×10² bacterial cells/ml for some models. Many Bacillus isolates were isolated, and the cells that formed the spores appeared in rods of different sizes, and the shape and location of the spores differed from the mother cells, where the isolate Bacillus species appeared in small-sized cells with a medial cylindrical spore and in the form of thin rod-shaped cells with a cylindrical spore. The spores are large, medium in size, and from the mother cell, while the sporophyte took a sub-terminal position in isolate Bacillus spp. The sporophyte appeared in a barrel shape, the average of the parent cell in the isolate, and the spheroid was mediated by the parent cell, and the cells of the isolate were characterized by their small size and the location of the sporophyte under the two ends where the most isolated were Bacillus, Enterobacteriaceae species. Conclusion Several types of bacteria accompanying mosquitoes were isolated, Bacillus spp., Staphylococcus, and Streptococcus faecalis, which cause many diseases in humans and need molecular diagnosis to confirm the isolated species more accurately.