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Segmented two-dimensional liquid chromatography. Proof of concept study
Kateřina Hrůzová1, Martina Nechvátalová1, Jiří Urban1
1Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
Journal of Chromatography. A
|February 2, 2023
Summary
This study introduces a novel two-dimensional liquid chromatography method, combining spatial and elution-based separations for enhanced analyte resolution. The technique successfully separated neurotransmitters in human urine, demonstrating its practical application.
Area of Science:
- Analytical Chemistry
- Chromatography
Background:
- Liquid chromatography separation can be defined by space or time.
- Two-dimensional liquid chromatography (2D-LC) offers enhanced separation capabilities.
- Online space x time coupling in 2D-LC is experimentally challenging.
Purpose of the Study:
- To develop a novel 2D-LC method combining spatial and time-based separations.
- To create an online, comprehensive analyte transfer system between dimensions.
- To apply the developed method for neurotransmitter analysis in human urine.
Main Methods:
- Utilized a 2D-LC system with serially coupled capillary columns and 2-position 6-port valves.
- Employed reversed-phase chromatography in the first (spatial) dimension and HILIC in the second (time-based) dimension.
- Developed and validated a theoretical model for the 2D separation.
Main Results:
- Successfully developed a novel 2D-LC method combining space and time separations.
- Demonstrated online, comprehensive transfer of analytes between dimensions.
- Achieved separation of neurotransmitters in human urine using the developed 2D-LC system.
Conclusions:
- The developed 2D-LC method offers a unique approach to chromatographic separation.
- The combination of spatial and time-based dimensions provides enhanced analytical power.
- This method shows promise for complex biological sample analysis, such as neurotransmitter profiling.
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