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Updated: Aug 11, 2025

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
Published on: February 23, 2018
Next-Generation Diagnostics for Pathogens
1Department of Comparative Pathobiology and Molecular Section Head, Animal Disease Diagnostic Laboratory, Purdue University College of Veterinary Medicine, 406 South University St., West Lafayette, IN 47907-2065, USA.
Abstract:
Next-generation sequencing (NGS) was initially developed to aid sequencing of the human genome. This molecular method is cost effective for sequencing and characterizing genomes, not only those of humans or animals but also those of bacteria and other pathogens. However, rather than sequencing a single organism, a targeted NGS method can be used to specifically amplify pathogens of interest in a clinical sample for detection and characterization by sequencing. Targeted NGS is an ideal method for ruminant syndromic testing due to its ability to detect a variety of pathogens in a sample with a single test.
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