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Related Experiment Video

Updated: Aug 11, 2025

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

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Published on: February 23, 2018

Next-Generation Diagnostics for Pathogens.

Rebecca P Wilkes¹

¹Department of Comparative Pathobiology and Molecular Section Head, Animal Disease Diagnostic Laboratory, Purdue University College of Veterinary Medicine, 406 South University St., West Lafayette, IN 47907-2065, USA.

The Veterinary Clinics of North America. Food Animal Practice

|February 2, 2023

Summary

This summary is machine-generated.

Targeted next-generation sequencing (NGS) offers a cost-effective method for detecting diverse pathogens in ruminant syndromic testing. This approach enables simultaneous identification and characterization of multiple disease-causing agents within a single clinical sample.

Keywords:

NGS infectious diseases Next-generation sequencing pathogen detection Ruminant molecular diagnostics Targeted NGS

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Area of Science:

Veterinary Medicine
Genomics
Molecular Diagnostics

Background:

Next-generation sequencing (NGS) is a powerful molecular method initially developed for human genome sequencing.
NGS technology provides cost-effective genome sequencing and characterization for various organisms, including bacteria and pathogens.
Traditional methods often require separate tests for each potential pathogen.

Purpose of the Study:

To evaluate targeted NGS as a method for ruminant syndromic testing.
To demonstrate the capability of targeted NGS in detecting and characterizing multiple pathogens simultaneously.

Main Methods:

Utilized a targeted next-generation sequencing approach.
Focused on amplifying specific pathogens of interest within clinical samples.
Employed sequencing for pathogen detection and characterization.

Main Results:

Targeted NGS allows for the detection of a variety of pathogens in a single clinical sample.
This method is suitable for comprehensive syndromic testing in ruminants.

Conclusions:

Targeted NGS is an ideal molecular diagnostic tool for ruminant syndromic testing.
The method provides a single-test solution for identifying multiple pathogens, improving diagnostic efficiency.