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Updated: Aug 11, 2025

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
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Non-canonical functions of spliceosome components in cancer progression

Olga M Ivanova1,2,3, Ksenia S Anufrieva4,5, Anastasia N Kazakova5,6

  • 1Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, 119435, Russian Federation. sci.olga.ivanova@gmail.com.

Cell Death & Disease
|February 2, 2023
PubMed

Insights

Dysregulation of pre-mRNA splicing factors impacts cancer progression through both splicing-dependent and independent mechanisms. Targeting spliceosome components offers promising anticancer therapeutic strategies.

Area of Science:

  • Molecular Biology
  • Cancer Biology
  • Genetics

Background:

  • Dysregulation of pre-messenger RNA (pre-mRNA) splicing is a hallmark of cancer.
  • Splicing machinery components are frequently altered in cancer cells.
  • Emerging evidence shows spliceosome components influence cellular processes independently of splicing.

Purpose of the Study:

  • To analyze the impact of splicing factor knockdown on genes related to cancer hallmarks.
  • To review non-canonical functions of splicing factors in cancer progression.
  • To explore spliceosome-targeted anticancer therapies.

Main Methods:

  • Analysis of open-source data on splicing factor knockdown in human cells.
  • Literature review of splicing factors' roles in cancer progression and intercellular communication.
  • Discussion of autoregulatory mechanisms and therapeutic targeting strategies.

Main Results:

  • Splicing factor knockdown affects expression and splicing of genes involved in proliferation, migration, cell cycle, DNA repair, and cell death.
  • Splicing factors exhibit non-canonical functions crucial for cancer progression.
  • Intercellular communication and autoregulation play roles in spliceosome component homeostasis.

Conclusions:

  • Spliceosome proteins are critical regulators in cancer, acting through both canonical and non-canonical pathways.
  • Understanding these roles is vital for developing novel anticancer therapies.
  • Targeting spliceosome components presents a promising avenue for cancer treatment.

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