Regulation of Pancreatic TXNIP-Insulin Expression Levels after Bariatric Surgery Using Diabetic Rodent Model
Jason Widjaja1,2, Yuxiao Chu1, Jian Wang1
1Department of Gastrointestinal Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou Jiangsu 221002, China.
Biomed Research International
|February 3, 2023
Summary
Bariatric surgery reduced pancreatic thioredoxin-interacting protein (TXNIP) and increased insulin expression in diabetic rats. These changes in TXNIP, miR-204, and MafA may be key to diabetes remission after surgery.
Area of Science:
- Endocrinology
- Metabolic Surgery
- Molecular Biology
Background:
- Thioredoxin-interacting protein (TXNIP) is implicated in glucose homeostasis.
- Bariatric surgery is effective for type 2 diabetes remission.
- The role of TXNIP in bariatric surgery's metabolic effects is unclear.
Purpose of the Study:
- To investigate the impact of bariatric surgery on pancreatic TXNIP and insulin expression.
- To determine if bariatric surgery alters TXNIP levels, a potential glucose control factor.
Main Methods:
- Diabetic rat models were used to assess pancreatic TXNIP regulation.
- Rats underwent gastric bypass, pair-feeding, or sham surgery.
- Oral glucose tolerance tests, insulin tolerance tests, and hormonal analyses were performed.
Main Results:
- Gastric bypass significantly decreased pancreatic TXNIP mRNA and protein levels.
- Insulin mRNA and protein expression were significantly higher post-gastric bypass.
- MicroRNA-204 (miR-204) and MafA expression levels were altered in the gastric bypass group.
Conclusions:
- Bariatric surgery improves glucose control by regulating pancreatic TXNIP, miR-204, and MafA.
- These molecular changes may contribute to diabetes remission following bariatric surgery.
