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Non-invasive Skeletal Muscle Quantification in Small Animals Using Micro-computed Tomography
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Three-dimensional mechanical characterization of murine skeletal muscle using quantitative micro-elastography.

Erin M Lloyd1,2, Matt S Hepburn3,4,2, Jiayue Li3,4,5

  • 1Department of Anatomy, Physiology and Human Biology, School of Human Sciences, The University of Western Australia, 35 Stirling Highway, Perth, Western Australia, 6009, Australia.

Biomedical Optics Express
|February 3, 2023
PubMed
Summary

Quantitative micro-elastography revealed significant decreases in skeletal muscle elasticity in aged, dysferlin-deficient mice. This finding offers insights into muscular dystrophy progression and age-related muscle changes.

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Area of Science:

  • Biomedical Engineering
  • Musculoskeletal Research
  • Biophysics

Background:

  • Skeletal muscle function relies on mechanical and structural properties, often altered by disease.
  • Understanding intermediate-scale tissue mechanics is crucial for insights into diseases like muscular dystrophies.

Purpose of the Study:

  • To characterize the micro-scale elasticity of ex vivo murine skeletal muscle in 3D using quantitative micro-elastography (QME).
  • To investigate the effects of aging and dysferlin deficiency on skeletal muscle elasticity.

Main Methods:

  • Quantitative micro-elastography (QME) was employed to assess micro-scale elasticity in 3D.
  • Murine quadriceps muscles (wild-type and dysferlin-deficient) were encapsulated in hydrogels to ensure high QME image quality.
  • Samples were analyzed at 3, 10, and 24 months of age.

Main Results:

  • A significant 77% decrease in elasticity was observed in dysferlin-deficient quadriceps compared to wild-type at 24 months.
  • This highlights age- and disease-related alterations in skeletal muscle mechanical properties.

Conclusions:

  • Quantitative micro-elastography is effective for characterizing 3D micro-scale elasticity in whole skeletal muscles.
  • Dysferlin deficiency leads to substantial age-dependent reductions in muscle elasticity, relevant to understanding dysferlinopathy.