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Coarse-scale optoretinography (CoORG) with extended field-of-view for normative characterization.

Xiaoyun Jiang1,2, Teng Liu1,3, Vimal Prabhu Pandiyan1

  • 1Department of Ophthalmology, University of Washington School of Medicine, Seattle, WA 98109, USA.

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|February 3, 2023
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A new coarse-scale optoretinography (CoORG) system without adaptive optics enables rapid, wide-field imaging of light-evoked retinal activity. This non-adaptive optics approach demonstrates high repeatability and agreement with existing methods, paving the way for clinical applications.

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Area of Science:

  • Ophthalmology
  • Biomedical Engineering
  • Retinal Imaging

Background:

  • Optoretinography (ORG) is a sensitive biomarker for retinal function.
  • Adaptive optics (AO) ORG provides cellular-scale resolution but limits field-of-view (FOV).
  • AO-ORG faces challenges with patient mobility and optical aberrations.

Purpose of the Study:

  • To develop a coarse-scale ORG (CoORG) system without AO for extended FOV imaging.
  • To assess the feasibility and repeatability of CoORG in healthy subjects.
  • To compare CoORG with AO-OCT based ORG.

Main Methods:

  • Developed a line-scan spectral domain OCT system with high volume rates (32 Hz).
  • Acquired 5.5-degree wide OCT volumes using CoORG after dark adaptation and bleaching stimuli.
  • Calculated stimulus-evoked optical phase change (ΔOPL) and assessed its variation with eccentricity.

Main Results:

  • CoORG achieved rapid acquisition (up to 32 Hz) over a 5.5-degree FOV.
  • High trial-to-trial repeatability of ΔOPL was observed across subjects and eccentricities.
  • CoORG showed excellent agreement with AO-OCT ORG, with response amplitude decreasing with eccentricity.

Conclusions:

  • CoORG offers a promising, non-AO approach for widespread clinical ORG application.
  • The system enables normative characterization of cone ORG response in healthy eyes.
  • Photon density and outer segment length explain variations in ORG characteristics.