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Integrated Signal Amplification on a Fiber Optic SPR Sensor Using Duplexed Aptamers.

Annelies Dillen1, Claudia Scarpellini1, Woud Daenen1

  • 1Department of Biosystems─Biosensors Group, KU Leuven, Willem de Croylaan 42, Box 2428, 3001Leuven, Belgium.

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Summary

This study introduces a novel fiber optic surface plasmon resonance (FO-SPR) biosensor using cis-duplexed aptamers and gold nanoparticles for sensitive detection of low molecular weight targets without complex steps.

Keywords:
biosensingduplexed aptamersfiber optic surface plasmon resonancegold nanoparticlesintegrated signal generation

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Area of Science:

  • Biosensing
  • Nanotechnology
  • Biomolecular interactions

Background:

  • Fiber optic surface plasmon resonance (FO-SPR) biosensors are effective for biomolecular interaction analysis and target detection.
  • Current FO-SPR methods often require multistep processes and external reagents for low molecular weight targets, increasing complexity and cost.
  • Continuous monitoring is hindered by the limitations of existing FO-SPR bioassays.

Purpose of the Study:

  • To develop an enhanced FO-SPR biosensor for sensitive detection of low molecular weight targets.
  • To overcome the limitations of multistep processes and external reagents in FO-SPR bioassays.
  • To enable continuous biosensing applications using FO-SPR technology.

Main Methods:

  • Implementation of cis-duplexed aptamers (DAs) on FO-SPR sensors for conformational change upon target binding.
  • Utilizing gold nanoparticles (AuNPs) covalently conjugated to the sensor for signal amplification via spatial redistribution.
  • Optimization of gold fiber coating thickness to enhance resonance conditions between FO-SPR sensors and AuNPs.

Main Results:

  • Achieved a 3-fold signal amplification by optimizing gold coating thickness for enhanced resonance.
  • Demonstrated specific and quantitative detection of an ssDNA target using DA-based FO-SPR sensors with a 230 nM detection limit.
  • Confirmed the reversibility of AuNP redistribution, a key feature for continuous measurements.

Conclusions:

  • The developed DA-based FO-SPR bioassay offers a simplified and sensitive method for detecting low molecular weight targets.
  • The covalent conjugation of AuNPs and aptamer-induced signal amplification eliminate the need for multistep processes and external reagents.
  • This innovative approach paves the way for advanced, continuous FO-SPR-based biosensing applications.