Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

RACE - Rapid Amplification of cDNA Ends

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

Environmental Applications of Microorganisms

Environmental Applications of Microorganisms

Microorganisms play a pivotal role in maintaining ecosystem balance by recycling essential elements such as carbon, nitrogen, and phosphorus, as well as supporting processes like bioremediation, wastewater treatment, and biofuel production.Microbes in Elemental CyclesIn the carbon cycle, microorganisms decompose organic matter, releasing carbon dioxide via aerobic respiration. This carbon dioxide is subsequently used by photosynthetic organisms to synthesize organic compounds, closing the...

Applications of Molecular Taxonomy

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Single-cell sequencing reveals unexpected genetic diversity among <i>Bodo</i> spp. flagellates and their bacterial endosymbionts.

Microbial genomics·2026

Same author

Partial mitochondrial genome of the enigmatic Bermuda fireworm Odontosyllis enopla Verrill, 1900 (Annelida, Syllidae, Eusyllinae) and its phylogenetic implications.

ZooKeys·2026

Same author

The genome sequence of Artibeus intermedius (Chiroptera, Phyllostomidae, Stenodermatinae; J. A. Allen, 1897).

Wellcome open research·2025

Same author

Comparative single-cell genomics of two uncultivated <i>Naegleria</i> species harboring <i>Legionella</i> cobionts.

mSphere·2025

Same author

AI for difficult herbarium specimens: identification of peat mosses (subgenus Sphagnum) without dissection.

The New phytologist·2025

Same author

The genome sequence of <i>Artibeus lituratus</i> (Chiroptera, Phyllostomidae, Stenodermatinae; Olfers, 1818).

Wellcome open research·2025

Search research articles

Related Experiment Video

Updated: Jun 27, 2026

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

Published on: November 25, 2016

An Environmental DNA Primer for Microbial and Restoration Ecology.

Michael Tessler^1,2,3, Seth W Cunningham^4,5, Melissa R Ingala⁶

¹Department of Biology, St. Francis College, Brooklyn, NY, USA. mtessler@sfc.edu.

Microbial Ecology

|February 3, 2023

Summary

Environmental DNA (eDNA) sequencing offers powerful tools for microbial ecologists. This review details essential eDNA methods for studying microbial communities and their application in ecological restoration.

Keywords:

Metabarcoding Metagenomics Microbiome Restoration ecology eDNA eRNA

More Related Videos

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Published on: November 5, 2020

Microbial DNA Analysis in the Field Using a Biological Extraction Field Kit and a Field qPCR Unit

Microbial DNA Analysis in the Field Using a Biological Extraction Field Kit and a Field qPCR Unit

Published on: January 2, 2026

Related Experiment Videos

Last Updated: Jun 27, 2026

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

Published on: November 25, 2016

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Published on: November 5, 2020

Microbial DNA Analysis in the Field Using a Biological Extraction Field Kit and a Field qPCR Unit

Microbial DNA Analysis in the Field Using a Biological Extraction Field Kit and a Field qPCR Unit

Published on: January 2, 2026

Area of Science:

Microbial Ecology
Environmental Genomics

Background:

Environmental DNA (eDNA) sequencing, initially developed for microbial studies, has advanced significantly with metabarcoding and metagenomics.
Modern eDNA approaches are increasingly utilized for analyzing diverse microbial assemblages (bacteria, archaea, protists, fungi, viruses).

Purpose of the Study:

To provide a foundational guide to eDNA methods for microbial ecologists new to DNA-based techniques.
To highlight the benefits and specific considerations of eDNA for ecological restoration.

Main Methods:

The review covers key aspects of eDNA methodology, including study design, sample collection and storage.
It discusses the selection of appropriate sequencing technologies, laboratory protocols, and essential equipment.
Basic bioinformatic tools relevant to eDNA analysis are also introduced.

Main Results:

The review synthesizes current eDNA practices pertinent to microbial ecology.
It emphasizes the growing accessibility and power of eDNA tools due to technological advancements and cost reductions.

Conclusions:

eDNA methods are rapidly evolving, presenting a learning curve for researchers.
This review serves as a crucial starting point for microbial ecologists seeking to implement eDNA techniques, particularly in restoration ecology.