Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

883
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
883
Chemotaxis in E. coli01:27

Chemotaxis in E. coli

649
Chemotaxis in Escherichia coli is a sensory-driven motility mechanism that enables bacteria to navigate chemical gradients, moving toward beneficial environments while avoiding harmful conditions. This process relies on a signal transduction system integrating external chemical cues with flagellar motor control.Chemoreceptors and Signal DetectionE. coli detects chemical gradients through methyl-accepting chemotaxis proteins (MCPs), which are membrane-bound chemoreceptors that sense attractants...
649
Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

4.3K
Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Disease activity and damage accrual during the early disease course in a multinational inception cohort of patients with systemic lupus erythematosus.

Lupus·2010
Same author

Bacteraemia with Campylobacter jejuni: no association with the virulence genes iam, cdtB, capA or virB.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2010
Same author

Emerging evidence for Q fever in humans in Denmark: role of contact with dairy cattle.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2009
Same author

Mortality in HIV-infected injection drug users with active vs cleared hepatitis C virus-infection: a population-based cohort study.

Journal of viral hepatitis·2009
Same author

Bacteraemia as a result of Campylobacter species: a population-based study of epidemiology and clinical risk factors.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2009
Same author

Seasonal variation in short-term mortality after surgery for colorectal cancer?

Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2009

Related Experiment Video

Updated: Jan 10, 2026

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages
09:57

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

Published on: August 12, 2019

10.1K

Antibiotics and human monocyte function. I. Chemotaxis.

H Nielsen1

  • 1Department of Clinical Microbiology, Statens Seruminstitut, Rigshospitalet, Copenhagen, Denmark.

Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology
|October 1, 1987
PubMed
Summary
This summary is machine-generated.

Most common antibacterial drugs do not affect human monocyte chemotaxis at normal doses. High concentrations of tetracycline, trimethoprim, and fusidic acid showed some inhibition in vitro.

More Related Videos

Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo
08:38

Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo

Published on: October 30, 2017

7.7K
Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions
09:41

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Published on: October 17, 2017

13.8K

Related Experiment Videos

Last Updated: Jan 10, 2026

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages
09:57

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

Published on: August 12, 2019

10.1K
Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo
08:38

Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo

Published on: October 30, 2017

7.7K
Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions
09:41

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Published on: October 17, 2017

13.8K

Area of Science:

  • Immunology
  • Pharmacology

Background:

  • Monocyte chemotaxis is crucial for immune response.
  • Antibacterial drugs can potentially modulate immune cell function.
  • Understanding drug effects on monocytes is important for clinical practice.

Purpose of the Study:

  • To investigate the in vitro effects of 13 common antibacterial drugs on human monocyte chemotaxis.
  • To determine if therapeutic concentrations of these drugs impact monocyte migration.
  • To predict the in vivo effects of these drugs on monocyte function.

Main Methods:

  • Human blood monocytes were isolated.
  • Monocyte chemotaxis was assessed in vitro.
  • The influence of 13 antibacterial drugs was evaluated at various concentrations.

Main Results:

  • Tetracycline, trimethoprim, and fusidic acid significantly inhibited monocyte chemotaxis at high concentrations.
  • Normal therapeutic concentrations of these three drugs showed insignificant inhibition.
  • Benzylpenicillin, ampicillin, tobramycin, chloramphenicol, metronidazole, rifampicin, clindamycin, sulfamethoxazole, cefotaxime, and ofloxacin did not alter monocyte migration.

Conclusions:

  • At normal therapeutic dosages, the tested antibacterial drugs are unlikely to affect in vivo monocyte chemotaxis.
  • High concentrations of certain antibiotics may impact monocyte function, warranting further investigation.
  • The study provides valuable data for clinicians regarding the immunomodulatory potential of common antibacterial agents.