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Updated: Aug 11, 2025
Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
Published on: April 18, 2019
An HLA-homozygous haplobank resource to promote safer cell therapies
1International Stem Cell Banking Initiative, 2 High Street, Barley SG88HZ, UK; National Stem Cell Resource Centre, Institute of Zoology, Chinese Academy of Sciences, Beijing 100190, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China; SSCBio Ltd, 2 High Street, Barley, Hertfordshire SG88HZ, UK.
Insights
Researchers created a bank of human induced pluripotent stem cells (iPSCs) from donors. This HLA-homozygous iPSC bank benefits regenerative medicine and allogeneic transplantation for a significant portion of the Japanese population.
Area of Science:
- Regenerative Medicine
- Stem Cell Biology
- Immunology
Background:
- Generating donor-derived induced pluripotent stem cells (iPSCs) for allogeneic transplantation presents a significant hurdle in regenerative medicine.
- HLA compatibility is crucial for successful transplantation to minimize immune rejection.
Purpose of the Study:
- To establish a human induced pluripotent stem cell (iPSC) bank with HLA homozygosity.
- To assess the coverage of this haplobank within the Japanese population.
- To outline manufacturing considerations for quality and safety.
Main Methods:
- Generation of induced pluripotent stem cells (iPSCs) from donor cells.
- HLA typing and homozygosity analysis for cell line selection.
- Population genetics analysis to determine coverage.
Main Results:
- Establishment of an HLA-homozygous iPSC haplobank.
- The haplobank covers approximately 40% of the Japanese population.
- Detailed quality and safety considerations for manufacturing were described.
Conclusions:
- The developed HLA-homozygous iPSC haplobank is a valuable resource for allogeneic transplantation in Japan.
- This resource addresses a key challenge in regenerative medicine, potentially improving transplant outcomes.
- Manufacturing protocols emphasize quality and safety for clinical application.
Abstract:
The generation of donor-derived induced pluripotent stem cells (iPSCs) for allogeneic transplantation is a major challenge in regenerative medicine. Yoshida et al. now report on the establishment of an HLA-homozygous haplobank of iPSCs that covers approximately 40% of the Japanese population and describe quality and safety considerations for manufacturing.
