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Related Concept Videos

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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Updated: Aug 11, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

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RNA m6A methylation across the transcriptome.

Erdem Sendinc1, Yang Shi2

  • 1Stem Cell Program, Division of Hematology/Oncology, Boston Children's Hospital, Boston, MA 02115, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.

Molecular Cell
|February 3, 2023
PubMed
Summary
This summary is machine-generated.

N6-methyl adenosine (m6A) is a widespread RNA modification in mammals, found on both coding and non-coding RNAs. Recent research highlights its nuclear roles in gene expression and chromatin regulation, mediated by conserved enzymes.

Keywords:
ERVIAPMETTL14METTL16METTL3METTL4METTL5PCIF1RNA modificationsZCCHC4epitranscriptomicsm6Am6Am

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Area of Science:

  • Molecular Biology
  • Epigenetics
  • Biochemistry

Background:

  • RNA modifications are crucial for gene regulation.
  • N6-methyl adenosine (m6A) is a highly abundant epitranscriptomic mark in mammalian cells.
  • m6A is found on various RNA types, including protein-coding and non-coding RNAs.

Purpose of the Study:

  • To review the current understanding of RNA m6A modification.
  • To highlight the nuclear roles of m6A in chromatin states and gene expression.
  • To discuss the enzymes responsible for m6A methylation and their substrates.

Main Methods:

  • Literature review of recent studies on RNA m6A.
  • Analysis of evolutionary conservation of m6A methyltransferases.
  • Examination of m6A's involvement in biological processes.

Main Results:

  • m6A modification is prevalent across diverse RNA species, including ribosomal RNAs and snRNAs.
  • Distinct, evolutionarily conserved enzymes catalyze m6A methylation.
  • m6A plays significant roles in regulating nuclear chromatin states and gene expression.

Conclusions:

  • RNA m6A is a fundamental regulatory mechanism with broad biological implications.
  • Further research is needed to elucidate the molecular mechanisms and functions of m6A.
  • Understanding m6A is critical for comprehending its role in health and disease.