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Sequestration of zearalenone using microorganisms blend in vitro
Vignesh B Nathan1, Hang Lu2, Nathan L Horn2
1Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN 47907, USA.
Letters in Applied Microbiology
|February 3, 2023
Summary
Two probiotic feed additives effectively sequestered zearalenone (ZEN), a mycotoxin causing reproductive issues in livestock. These supplements reduced ZEN levels by 45% and prevented its harmful effects on cell proliferation.
Area of Science:
- Mycology
- Toxicology
- Animal Science
Background:
- Zearalenone (ZEN) is an estrogenic mycotoxin from Fusarium species, posing significant reproductive health risks in livestock.
- Mycotoxin contamination is a major concern for animal agriculture, impacting animal health and productivity.
Purpose of the Study:
- To evaluate the efficacy of two commercial probiotic feed additives in sequestering ZEN.
- To determine the impact of probiotic treatment on ZEN's biological activity using cell-based assays.
Main Methods:
- Commercial probiotic blends were incubated with ZEN over time.
- Unbound ZEN was quantified using Enzyme-Linked Immunosorbent Assay (ELISA).
- ZEN's effects on MCF-7 cell proliferation were assessed to confirm sequestration.
Main Results:
- Probiotic treatments reduced ZEN concentration by approximately 45% (∼100 μg L-1).
- Probiotic blends prevented ZEN-induced MCF-7 cell proliferation, showing a 20%-28% reduction.
- ZEN and the probiotic mix were found to be non-toxic to MCF-7 cells.
Conclusions:
- Commercial probiotic feed supplements demonstrate potential for effective ZEN neutralization.
- Probiotic interventions may offer a viable strategy to mitigate the adverse effects of ZEN in animal feed.
- Further research could explore optimal probiotic combinations and dosages for mycotoxin management.
