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Related Experiment Videos

A versatile and sensitive method for measuring oxygen.

D F Wilson1, J M Vanderkooi, T J Green

  • 1Department of Biochemistry and Biophysics, Medical School, University of Pennsylvania, Philadelphia 19104.

Advances in Experimental Medicine and Biology
|January 1, 1987
PubMed
Summary
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Measuring oxygen concentration in water using phosphorescent molecules

Area of Science:

  • Analytical Chemistry
  • Photochemistry
  • Biomedical Sensing

Background:

  • Oxygen concentration is critical in aqueous environments.
  • Traditional methods like oxygen electrodes have limitations.
  • Optical methods based on luminescence quenching are susceptible to interference.

Purpose of the Study:

  • To introduce a novel method for measuring oxygen concentration in aqueous media.
  • To leverage the oxygen dependence of excited triplet state lifetimes of phosphorescent molecules.
  • To overcome limitations of existing oxygen measurement techniques.

Main Methods:

  • Utilizing the oxygen-dependent lifetime of the excited triplet state of phosphorescent molecules.
  • Measuring phosphorescence lifetimes across a wide dynamic range (microseconds to seconds).

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  • Employing phosphorescent probes for continuous oxygen monitoring.

    • Main Results:

    • Demonstrated insensitivity to optical interference common in other luminescence-based methods.
    • Achieved measurements at significantly lower oxygen concentrations than oxygen electrodes.
    • Enabled continuous measurement of oxygen concentrations from 10(-4) M to 10(-8) M.

    Conclusions:

    • Phosphorescence lifetime measurements offer a robust and sensitive method for determining oxygen concentration in aqueous solutions.
    • This technique provides a wider dynamic range and improved accuracy compared to existing methods.
    • The approach is suitable for continuous, low-concentration oxygen monitoring in various applications.