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Updated: Aug 11, 2025
Endotoxin Activity Assay for the Detection of Whole Blood Endotoxemia in Critically Ill Patients
Published on: June 24, 2019
Clinical relevance of leukocyte-associated endotoxins measured by semi-automatic synthetic luminescent substrate
Mari Terayama1, Gaku Takahashi2, Maria Nonoguchi1
1Department of Critical Care, Disaster and General Medicine, School of Medicine, Iwate Medical University, 2-1-1 Idaidori, Yahaba Town, Iwate, 028-3695, Japan.
Abstract:
A newly developed semi-automatic synthetic luminescence substrate (SALS) method for measuring endotoxin was compared with the existing turbidimetric kinetic assay (TKA) using leukocyte-rich plasma to verify its usefulness. As a result, the endotoxin levels by this method were higher than that by the existing assay in most specimens, and the time required for measurement was much shorter. In addition, the leukocyte-rich plasma endotoxin level minus the plasma endotoxin levels were named leukocyte-associated endotoxin, and these levels per one leukocyte were compared. As a result, those levels were highly correlated with the endotoxin measurement levels of leukocyte-rich plasma. The correlation coefficient of SALS method was superior to the existing TKA method, the endotoxin level by this method may be close to true endotoxin levels.
