Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

17.2K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.2K
CRISPR01:59

CRISPR

52.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.6K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

107
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
107
Homologous Recombination02:31

Homologous Recombination

50.8K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.8K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

72
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
72
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

79
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
79

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advanced Degradation and Remediation Strategies for Per- and Polyfluoroalkyl Substances (PFASs): Challenges and Future Perspectives.

Toxics·2026
Same author

High-sensitivity optical thermometry in CaWO<sub>4</sub>: Bi<sup>3+</sup>, Pr<sup>3+</sup> phosphors based on distinct thermal quenching behaviors.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same author

Potassium Biofortification of Quinoa Microgreens: A Holistic Enhancement of Yield, Nutritional Components, and Bioactive Content.

Plant foods for human nutrition (Dordrecht, Netherlands)·2026
Same author

Dual-wavelength LED synergy-tailored charge dynamics and reactive species modulation for a high-performance photocatalytic ceramic membrane reactor in complex water matrices.

Water research·2026
Same author

Therapeutic and prognostic value of repeat transurethral resection for high-grade Ta bladder cancer: a propensity score matching analysis.

American journal of cancer research·2026
Same author

Multimodal neuroimaging alterations in schizophrenia model rats and the modulatory effects of risperidone: a pilot study.

Frontiers in psychiatry·2026
Same journal

[Recent progress and policy recommendations for biomanufacturing technology in China].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology·2026
Same journal

[CRISPR/Cas9-mediated knockout of the <i>Soc</i> gene in T4 bacteriophage and mutant construction].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology·2026
Same journal

[High-throughput fluorescence-activated droplet sorting of polyethylene terephthalate hydrolases based on fluorescent nanoparticle biosensors].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology·2026
Same journal

[Machine learning-optimized solid-state fermentation of non-grain biomass for enhanced feed protein production].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology·2026
Same journal

[Growth and metabolic characteristics and kinetic modeling of MDCK adherent cells cultured in basket bioreactors].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology·2026
Same journal

[Metabolic engineering of <i>Saccharomyces cerevisiae</i> for <i>de novo</i> biosynthesis of gibberellin A<sub>3</sub>].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology·2026
See all related articles

Related Experiment Video

Updated: Aug 11, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.3K

[CRISPR-based molecular diagnostics: a review].

Wenjun Sun1, Xingxu Huang1,2, Xinjie Wang3,2

  • 1School of Life Science and Technology, Shanghai Tech University, Shanghai 201210, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|February 4, 2023
PubMed
Summary
This summary is machine-generated.

CRISPR-based nucleic acid detection offers rapid, sensitive, and cost-effective molecular diagnostics. This technology is revolutionizing disease detection, from infectious agents like COVID-19 to cancer mutations and food safety.

Keywords:
CRISPRCRISPR-based detectiongene editingmolecular detectionnucleic acid detectionpoint-of-care testing (POCT)

More Related Videos

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

430
Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

364

Related Experiment Videos

Last Updated: Aug 11, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.3K
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

430
Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

364

Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Gene Editing

Background:

  • The COVID-19 pandemic underscored the critical need for rapid and sensitive disease detection technologies.
  • CRISPR/Cas gene editing has emerged as a transformative tool in biotechnology.
  • Molecular diagnostics are essential for disease prevention, control, and public health.

Approach:

  • This review summarizes recent advancements in CRISPR-based nucleic acid detection systems.
  • It explores the application of CRISPR technology in various diagnostic fields.
  • The review also discusses future perspectives on integrating CRISPR diagnostics with artificial intelligence for intelligent systems.

Key Points:

  • CRISPR-based nucleic acid detection is characterized by its speed, accuracy, sensitivity, and cost-effectiveness.
  • This technology is revolutionizing molecular diagnostics, offering a powerful alternative to traditional methods.
  • Applications span infectious disease detection (e.g., COVID-19), genetic disease diagnosis, cancer mutation identification, and food safety analysis.

Conclusions:

  • CRISPR-based nucleic acid detection systems represent a significant leap forward in molecular diagnostics.
  • The versatility and efficiency of CRISPR technology enable broad applications across healthcare and beyond.
  • Future developments may involve intelligent diagnostic platforms combining CRISPR with AI for enhanced disease management.