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Related Concept Videos

EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

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Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
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Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery
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Engineered Platforms for Maturing Pluripotent Stem Cell-Derived Liver Cells for Disease Modeling.

Yang Yuan1, Kristen Cotton1, Dinithi Samarasekera1

  • 1Department of Biomedical Engineering, University of Illinois at Chicago, Chicago, Illinois.

Cellular and Molecular Gastroenterology and Hepatology
|February 4, 2023
PubMed
Summary

Advanced techniques significantly improve human hepatocyte-like cells (HLCs) derived from pluripotent stem cells. These enhanced HLCs offer a scalable, patient-specific model for studying liver diseases and developing new therapeutics, reducing reliance on animal models.

Keywords:
microfluidicsmicropatterningorganoidsspheroidssynthetic biology

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Area of Science:

  • Regenerative Medicine
  • Hepatology
  • Stem Cell Biology

Background:

  • Liver diseases pose a significant global health burden, impacting mortality and morbidity.
  • Existing in vitro human liver models face limitations due to primary cell scarcity, donor variability, and species-specific differences.
  • Induced pluripotent stem cell (iPSC)-derived hepatocyte-like cells (HLCs) offer a renewable resource but typically exhibit immature phenotypes.

Purpose of the Study:

  • To present advanced techniques for enhancing the maturation of human hepatocyte-like cells (HLCs).
  • To discuss the application of these advanced HLC models in studying various liver diseases.
  • To highlight future directions for improving pluripotent stem cell-derived liver models in preclinical research and therapy.

Main Methods:

  • Utilizing advanced techniques including protein micropatterning, controlled spheroids, organoids, 3D bioprinting, microfluidic devices, and synthetic biology.
  • Precisely controlling cell-cell interactions, spatial organization, and microenvironment factors to promote HLC maturation.
  • Characterizing the design features and performance of these advanced HLC maturation platforms.

Main Results:

  • Advanced techniques significantly improve the maturation of HLCs derived from pluripotent stem cells.
  • These enhanced HLCs provide a more physiologically relevant model for studying liver diseases.
  • The developed platforms demonstrate potential for modeling diverse liver pathologies and disease mechanisms.

Conclusions:

  • Pluripotent stem cell-derived liver models, when matured using advanced techniques, offer a scalable and patient-specific alternative to traditional models.
  • These enhanced HLCs can accelerate preclinical drug development, reduce animal usage, and aid in discovering novel therapeutics.
  • Further advancements in HLC maturation hold promise for cell-based therapies for end-stage liver failure.