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Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples
Published on: November 23, 2016
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The rapid-tome, a 3D-printed microtome, and an updated hand-sectioning method for high-quality plant sectioning
David J Thomas1,2, Jordan Rainbow2, Laura E Bartley3
1Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, 73019, USA.
Plant Methods
|February 4, 2023
Summary
A new hand-sectioning method and the novel, 3D-printable Rapid-Tome device allow for high-quality plant sample sectioning. This accessible technology enables accurate anatomical studies for researchers and students across various plant types.
Area of Science:
- Plant anatomy
- Microscopy
- Botanical research
Background:
- Accurate microscopic analysis of plant anatomy requires high-quality tissue sections.
- Traditional sectioning methods are limited by sample hardness and consistency.
Purpose of the Study:
- To present a novel, cost-effective method for sectioning diverse plant samples.
- To improve accessibility of plant anatomical studies for research and education.
Main Methods:
- Development of a 3D-printable microtome, the "Rapid-Tome", for sectioning plant stems and roots.
- A complementary hand-sectioning technique for softer herbaceous tissues.
- Utilizing common hardware parts for Rapid-Tome assembly.
Main Results:
- The Rapid-Tome successfully sectioned soft and tough plant samples, including woody stems and fibrous roots.
- Comparative analysis revealed significant differences in seasonal xylem vessel area in sycamore.
- High school students produced adequate sections for analysis within an hour.
Conclusions:
- The Rapid-Tome offers a cost-effective, safe, and user-friendly alternative to traditional microtomes.
- This technology enhances the production of high-quality plant sections for research, education, and outreach.
- The device's portability and ease of use make thin section production accessible with minimal training.
