Optimization of aflatoxin B₁ removal efficiency of DNA by resonance light scattering spectroscopy

In this paper, firstly, the resonance light scattering spectra of aflatoxin B₁ (AFB₁) and DNA were measured by resonance light scattering spectroscopy (RLS), and the DNA binding saturation value (DBSV) of AFB₁ was calculated from their spectral results. Then the interaction intensity between DNA and AFB₁ and the effects of some external factors on the interaction between DNA and AFB₁ were evaluated by corresponding DBSVs, so as to establish and optimize a way for removing AFB₁ by DNA. DBSV of AFB₁ was 2.04 at 30℃ and pH 7.40. However, after adding sodium ion, calcium ion, vitamin E, vitamin C and D-glucose, DBSV of AFB₁ was changed to 2.72, 3.17, 2.67, 1.68 and 1.33 respectively. Correspondingly, the removal efficiency of AFB₁ by DNA was changed from 90.05% to 93.25%, 95.48%, 93.08%, 82.36% and 78.90% respectively. These results indicated that the external factors had a significant impact on the interaction between DNA and AFB₁. Among them, some factors enhanced the interaction between DNA and AFB₁, while some factors weakened the interaction between DNA and AFB1. The change of these external factors led to the corresponding changes in DBSV and the removal efficiency of AFB₁. DBSV of AFB₁ could really be used as an index to evaluate the intensity of the interaction between DNA and AFB₁, and to optimize the removal efficiency of AFB₁ by DNA. The experimental data also showed that the adsorption of AFB₁ to DNA was consistent with the pseudo-second-order kinetic model and the Freundlich isothermal model, was an exothermic and spontaneous process. All these results will give good references for establishing and optimizing a way of AFB₁ removal via DNA intercalation.