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Updated: Aug 11, 2025
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Published on: December 27, 2024
Carbon quantum dots as a nitric oxide donor can promote wound healing of deep partial-thickness burns in rats
Tao Tang1, Yingying Liu1, Peng Wang2
1Burn Institute of PLA, Department of Burn Surgery, the First Affiliated Hospital of Naval Medical University, Research Unit of key techniques for treatment of burns and combined burns and trauma injury, Chinese Academy of Medical Sciences, Shanghai, 200433, China.
Introduction:
In this study, a new carbon quantum dots-NO (CQDs-NO) that is based on spermidine trihydrochloride and can be used as a nitric oxide donor was prepared using a two-step hyperthermia-intermittent ultrasonic method, after which its healing effect on deep partial-thickness burn wounds was tested in rats.
Materials And Methods:
CQDs-NO were prepared by a two-step hyperthermia-intermittent ultrasonic method. NO-released rate and biocompatibility of CQDs-NO were tested. The biological functions of CQDs-NO were measured by scratch assay, Western blotting, histology, and transcriptome sequencing.
Results:
CQDs-NO with a concentration of 1 μg/mL and 5 μg/mL showed no cytotoxicity. CQDs-NO could release NO when co-cultured with cells or glutathione peroxidase. We also found that CQDs-NO promotes the biological processes such as angiogenesis, cell-substrate adhesion, extracellular matrix organization, cell migration, and wound healing in human umbilical vein endothelial cells (HUVEC). Additionally, CQDs-NO promoted wound healing of deep partial-thickness burn by enhancing vascularization, matrix deposition, as well as regulating the inflammatory reactions of wounds.
Conclusions:
CQDs-NO could be used as an alternative method for deep partial-thickness burn healing.
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