Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

426
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI spectrometry is widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.
The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix material. The...
426
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

5.6K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
5.6K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.6K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.6K
Mass Spectrometers01:16

Mass Spectrometers

5.8K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.8K
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

1.5K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.5K
Mass Spectrometry: Molecular Fragmentation Overview01:20

Mass Spectrometry: Molecular Fragmentation Overview

3.5K
The ionization of a molecule into a molecular ion inside the mass spectrometer causes instability in the molecule's structure due to the loss of an electron. This eventually leads to the fragmentation or breaking of some bonds in the molecule. The fragmentation occurs predominantly at specific bonds to yield relatively stable fragments.
One type of fragmentation pattern is the cleavage of a single bond in the molecular ion. The cleavage leads to a radical and a cation. The cleavage can...
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hydrogen-Deuterium Back-Exchange for High-Resolution Mass-Spectrometry-Based Metabolite Characterization.

Analytical chemistry·2026
Same author

Lipidomic signatures of CNS ischemic injury and their modulation by immunomodulatory hydrogels.

The Analyst·2026
Same author

A Convolutional Neural Network and Transfer Learning Approach for Accelerated Quantitative Mass Spectrometry Imaging.

Journal of mass spectrometry : JMS·2026
Same author

Manipulation of Gas-Phase Charge Inversion Ion/Ion Reaction Kinetics for Improved Phospholipid Identification in Imaging Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same author

Ozonolysis of Strontium-Adducted Phosphatidylcholines in the Gas Phase: Expanding the Isomeric Coverage of Ozone-Induced Dissociation.

Journal of the American Society for Mass Spectrometry·2026
Same author

Enhancing IR-MALDESI MSI Spatial Resolution Through Beam Constriction With a Ring-Actuated Iris.

Rapid communications in mass spectrometry : RCM·2026

Related Experiment Video

Updated: Aug 11, 2025

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI

Published on: March 24, 2016

9.6K

SMART: A data reporting standard for mass spectrometry imaging.

Ying Xi1,2, Alexandria L Sohn1, Alena N Joignant1

  • 1FTMS Laboratory for Human Health Research, Department of Chemistry, North Carolina State University, Raleigh, North Carolina, USA.

Journal of Mass Spectrometry : JMS
|February 5, 2023
PubMed
Summary
This summary is machine-generated.

Mass spectrometry imaging (MSI) data reporting lacks consistency, hindering comparisons. This tutorial introduces SMART, a unified system to standardize essential baseline information for improved MSI data interpretation and benchmarking.

Keywords:
data reporting standardsmass spectrometry imaging

More Related Videos

Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models
08:14

Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models

Published on: December 5, 2014

18.3K
Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

18.7K

Related Experiment Videos

Last Updated: Aug 11, 2025

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI

Published on: March 24, 2016

9.6K
Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models
08:14

Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models

Published on: December 5, 2014

18.3K
Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

18.7K

Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Clinical Research

Background:

  • Mass spectrometry imaging (MSI) is a powerful technique for spatial and quantitative ion analysis across various scientific fields.
  • Inconsistent data reporting practices among research groups and techniques impede the comparison of MSI data.
  • This inconsistency poses challenges for intra- and inter-laboratory data reproducibility and validation.

Purpose of the Study:

  • To propose a unified data reporting system, called SMART (Standardized Mass spectrometry data Reporting والتنسيق), for mass spectrometry imaging.
  • To establish a consistent format for reporting essential baseline information in MSI studies.
  • To facilitate easier understanding and benchmarking of MSI data across different studies and platforms.

Main Methods:

  • Development of the SMART reporting system based on common features across different MSI techniques.
  • Focus on capturing essential baseline parameters crucial for data interpretation.
  • The system is designed for simplicity and broad applicability, not exhaustive detail.

Main Results:

  • The SMART system provides a standardized framework for reporting MSI data.
  • It aims to improve the comparability of data within and between laboratories.
  • SMART enables clearer interpretation of MSI study parameters and outcomes.

Conclusions:

  • A uniform reporting system like SMART is necessary for effective communication of MSI data in publications and presentations.
  • Standardization enhances the ability to interpret MSI parameters and baseline results.
  • SMART offers a practical solution to address current inconsistencies in MSI data reporting.