Hsa_circ_0063329 inhibits prostate cancer growth and metastasis by modulating the miR-605-5p/tgif2 axis

Area of Science:

• Oncology
• Molecular Biology
• Genetics

Background:

• Circular RNAs (circRNAs) are critical regulators in various cancers.
• The specific roles and mechanisms of circRNAs in prostate cancer (PCa) progression and metastasis are not fully understood.

Purpose of the Study:

• To identify differentially expressed circRNAs in PCa.
• To elucidate the functional role and molecular mechanism of hsa_circ_0063329 in PCa proliferation and metastasis.

Main Methods:

• CircRNA microarray analysis to identify differentially expressed circRNAs.
• In vitro and in vivo functional assays to assess the role of hsa_circ_0063329.
• Bioinformatics, RNA pulldown, RNA-seq, and dual-luciferase reporter assays to determine the underlying molecular mechanism.

Main Results:

• Hsa_circ_0063329 was found to be significantly downregulated in PCa.
• Overexpression of hsa_circ_0063329 inhibited PCa cell progression, while its silencing promoted it.
• Hsa_circ_0063329 acts as a sponge for miR-605-5p, leading to the derepression of TG-interacting factor 2 (TGIF2) and inactivation of the TGF-β pathway.

Conclusions:

• Hsa_circ_0063329 inhibits PCa proliferation and metastasis through the miR-605-5p/TGIF2 axis.
• Targeting hsa_circ_0063329 presents a potential therapeutic strategy for aggressive PCa.