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Updated: Aug 11, 2025
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Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
Published on: January 12, 2020
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Expressions and Prognostic Values of Notch3 and DLL4 in Human Breast Cancer
1481873Shihezi University School of Medicine, Shihezi, Xinjiang, China.
Technology in Cancer Research & Treatment
|February 6, 2023
Summary
Notch3 and DLL4 protein expression promotes breast cancer progression and poor prognosis. Notch3 overexpression independently predicts poor outcomes, suggesting its potential as a prognostic biomarker.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Notch signaling is implicated in breast tumorigenesis.
- The prognostic significance of Notch3 and DLL4 in breast carcinoma remains underexplored.
Purpose of the Study:
- To investigate the expression and prognostic value of Notch3 and DLL4 in breast carcinoma.
- To analyze the correlation between Notch3/DLL4 expression and clinicopathological features and patient survival.
Main Methods:
- Retrospective study of 90 breast cancer tissues and 60 noncancerous tissues.
- Utilized Oncomine and UALCAN databases for expression analysis.
- Quantitative real-time PCR, western blotting, and immunohistochemistry were performed.
- Correlated protein expression with clinicopathological parameters and survival data.
Main Results:
- Notch3 and DLL4 expression were elevated in breast carcinoma, with Notch3 positively associated with DLL4.
- Both proteins correlated with advanced stage, high grade, and negative Her2 status.
- Overexpression of Notch3 and/or DLL4 was linked to reduced patient survival.
- Notch3 overexpression emerged as an independent predictor of poor prognosis.
Conclusions:
- The interaction between Notch3 receptor and DLL4 ligand accelerates breast cancer oncogenesis, progression, and poor prognosis.
- Notch3 protein shows potential as an independent prognostic biomarker for breast cancer patients.
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