Planar carbon electrodes for real-time quantification of hydrogen sulfide release from cells

A planar electrode system was developed to permit the real-time, selective detection of hydrogen sulfide (H₂S) from stimulated cells. Planar carbon electrodes were produced via stencil printing carbon ink through a laser cut vinyl mask. Electrodes were preconditioned using a constant potential amperometry methodology to prevent sensor drift resulting from elemental sulfur adsorption. Modification with a bilaminar coating (electropolymerized ortho-phenylenediamine and a fluorinated xerogel) facilitated high selectivity to H₂S. To demonstrate the biological application of this planar sensor system, H₂S released from 17β-estradiol-stimulated human umbilical vein endothelial cells (HUVECs) was quantified in situ in real-time. Stimulated HUVECs released sustained H₂S levels for hours before returning to baseline. Cellular viability assays demonstrated negligible cell cytotoxicity at the electrochemical potentials required for analysis.