Photosynthesis driven continuous hydrogen production by diazotrophic cyanobacteria in high cell density capillary...

Hydrogen (H₂) is a promising fuel in the context of climate neutral energy carriers and photosynthesis-driven H₂-production is an interesting option relying mainly on sunlight and water as resources. However, this approach depends on suitable biocatalysts and innovative photobioreactor designs to maximize cell performance and H₂ titers. Cyanobacteria were used as biocatalysts in capillary biofilm photobioreactors (CBRs). We show that biofilm formation/stability depend on light and CO₂ availabilityH₂ production rates correlate with these parameters but differ between Anabaena and Nostoc. We demonstrate that high light and corresponding O₂ levels influence biofilm stability in CBR. By adjusting these parameters, biofilm formation/stability could be enhanced, and H₂ formation was stable for weeks. Final biocatalyst titers reached up to 100 g l^-1 for N. punctiforme atcc 29133 NHM5 and Anabaena sp. pcc 7120 AMC 414. H₂ production rates were up to 300 µmol H₂ l^-1h^-1 and 3 µmol H₂ g_cdw^-1h^-1 in biofilms.