Related Experiment Video
Updated: Aug 11, 2025
A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
Published on: June 26, 2020
Survival and inactivation of hepatitis E virus on inanimate surfaces
J E Wißmann1, Y Brüggemann1, D Todt2
1Department for Molecular & Medical Virology, Ruhr University Bochum, Bochum, Germany.
Background:
Hepatitis E virus (HEV) is the most common cause of acute viral hepatitis, and mainly transmitted via faecal-oral contamination or consumption of contaminated food products. However, limited data on the surface stability and HEV sensitivity to chemical disinfectants are available.
Aim:
To establish an HEV-based carrier assay to evaluate its surface stability and the virucidal activity of nine surface disinfectants.
Methods:
A recently developed robust HEV-3 cell culture system for an HEV-based carrier assay.
Findings:
Alcohol-based disinfectants were insufficient to eliminate HEV infectivity, whereas disinfectants based on aldehyde, peracetic acid, oxygen, and/or quaternary ammonium inactivated HEV.
Conclusion:
These findings have strong implications for the recommendation of evidence-based hygiene guidelines to reduce HEV transmission.
