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Published on: November 7, 2013
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Novel opto-fluidic drug delivery system for efficient cellular transfection
Majid Layachi1,2,3, Anthony Treizebré1,2, Laurent Hay1
1Laboratoire Physique des Lasers, Atomes et Molécules - UMR 8523, Université de Lille, 59655, Villeneuve d'Ascq, France.
Journal of Nanobiotechnology
|February 7, 2023
Summary
A novel optofluidic method enhances intracellular drug delivery using gold nanoparticle photoporation. This technique achieves high cell transfection efficiency and viability, paving the way for clinical applications in gene therapy.
Area of Science:
- Biotechnology
- Nanotechnology
- Cell Biology
Background:
- Intracellular drug delivery is crucial for diagnostics and gene therapy.
- Current methods like non-viral carriers and physical techniques have limitations in yield, throughput, and cell viability.
- Gold nanoparticle (AuNP)-mediated photoporation offers a promising route for cell membrane permeabilization via laser-induced Vapor NanoBubble (VNB) generation.
Purpose of the Study:
- To develop an optofluidic method for efficient and non-contact gold nanoparticle photoporation.
- To overcome the limitations of existing photoporation techniques, particularly the issue of non-degradable gold nanoparticles in therapeutic cell engineering.
- To enable high-throughput, high-viability intracellular delivery for potential clinical translation.
Main Methods:
- Utilized hydrodynamic focusing in a multi-flow device to position gold nanoparticles near cells without direct contact.
- Employed laser-induced Vapor NanoBubble (VNB) generation for cell membrane photoporation.
- Integrated optofluidics for controlled delivery and high-throughput processing.
Main Results:
- Achieved high photoporation efficiency of [Formula: see text].
- Maintained significant cell viability with no substantial reduction.
- Demonstrated a high throughput ranging from [Formula: see text] to [Formula: see text].
Conclusions:
- The developed optofluidic method successfully enables gold nanoparticle photoporation with high efficiency and cell viability.
- This approach addresses the challenge of non-degradable nanoparticle accumulation in therapeutic cells.
- The technology shows promise for translating photoporation into clinical settings for producing genetically engineered therapeutic cells.
