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scMultiome analysis identifies embryonic hindbrain progenitors with mixed rhombomere identities
Yong-Il Kim1, Rebecca O'Rourke1, Charles G Sagerström1
1Section of Developmental Biology, Department of Pediatrics, University of Colorado Medical School, 12801 E. 17th Avenue, Aurora, CO 80045.
Biorxiv : the Preprint Server for Biology
|February 7, 2023
Summary
New zebrafish research reveals that early hindbrain segments contain mixed progenitor cells. These cells resolve into distinct rhombomeres, crucial for neural circuit development, through unique gene expression and chromatin profiles.
Area of Science:
- Developmental biology
- Neuroscience
- Genomics
Background:
- Rhombomeres are essential for embryonic hindbrain development and neural circuit formation.
- The molecular identities and formation mechanisms of individual rhombomeres remain incompletely understood.
Approach:
- Utilized single-cell multiome (scMultiome) analysis in zebrafish embryos.
- Examined gene expression and chromatin accessibility profiles across rhombomere development.
Key Points:
- Rhombomeres acquire distinct molecular identities between 10 and 13 hours post-fertilization.
- Early hindbrain progenitor populations (PHPDs) exhibit mixed molecular identities, not clear odd/even rhombomere characteristics.
- PHPDs are influenced by Fgf and RA signaling and represent prospective rhombomeres r2/r3, r4, and r5/r6.
Conclusions:
- Early hindbrain segmentation involves progenitor clusters with mixed cell identities.
- Resolution of these mixed transcription and chromatin states is critical for forming mature, distinct rhombomeres.
