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NFATC1 and NFATC2 expression patterns in human osteochondromas

Yuanyuan Wang1,2, Jiangdong Ren3, Guojin Hou4

  • 1Department of Stomatology, Xuanwu Hospital Capital Medical University, Beijing, China.

Heliyon
|February 7, 2023
PubMed
Abstract

Insights

Nuclear Factor of Activated T-cells (NFAT) signaling, specifically NFATc1 and NFATc2, appears suppressed in human osteochondromas. This contrasts with mouse models, suggesting a different role in human osteochondroma development.

Area of Science:

  • Molecular biology
  • Cell biology
  • Oncology
  • Orthopedics

Background:

  • Previous studies in mouse models indicated that NFATc1 and NFATc2 inhibit osteochondroma formation.
  • The role of NFAT signaling in human osteochondroma development remains largely unknown.
  • Osteochondromas are benign bone tumors often affecting adolescents and young adults.

Purpose of the Study:

  • To investigate the expression patterns of NFATc1 and NFATc2 in human osteochondroma samples.
  • To determine if NFAT signaling is involved in human osteochondrogenesis.
  • To compare NFATc1 expression in human osteochondromas with normal human periosteum and progenitors.

Main Methods:

  • Immunohistochemistry (IHC) was employed to analyze NFATc1 and NFATc2 expression in human osteochondromas.
  • IHC was used to map NFATc1 expression in the human periosteum.
  • Western blotting characterized NFATc1 expression in human periosteal progenitors compared to bone marrow stromal cells (BMSC).

Main Results:

  • NFATc1 and NFATc2 expression was largely undetectable in human osteochondroma chondrocytes.
  • A small subset of osteochondroma cells, particularly clonally grown chondrocytes, showed positive NFATc1 staining.
  • NFATc1 expression in the human periosteum was comparable to mouse periosteum, and highly enriched in periosteal progenitors versus BMSC.

Conclusions:

  • NFATc1 and NFATc2 are generally absent in human osteochondroma chondrocytes.
  • The observed expression pattern suggests NFAT signaling may be suppressed during human osteochondroma development.
  • Further research is needed to elucidate the precise role of NFAT signaling in human osteochondrogenesis.
Keywords:
Hereditary multiple exostosesNFATC1NFATC2OsteochondromaPeriosteal progenitorsPeriosteum

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