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Related Experiment Videos

[Intracellular ATP content and nonactin biosynthesis].

S Negrin, M V Nefelova, E M Gavrilova

    Antibiotiki I Meditsinskaia Biotekhnologiia = Antibiotics and Medical Biotechnology
    |August 1, 1987
    PubMed
    Summary

    Potassium orthophosphate influences antibiotic production in Str. chrysomallus var. macrotetrolidi. Optimal nonactin biosynthesis occurs when intracellular ATP, protein, and hemin levels are low, especially during declining ATP levels.

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    Area of Science:

    • Microbiology
    • Biochemistry
    • Industrial Biotechnology

    Background:

    • Streptomyces chrysomallus var. macrotetrolidi produces the macrolide antibiotic nonactin.
    • Intracellular metabolites like ATP and hemin play crucial roles in microbial growth and secondary metabolite production.
    • Phosphate availability is a key factor influencing microbial metabolism and biosynthesis.

    Purpose of the Study:

    • To investigate the impact of potassium orthophosphate on nonactin biosynthesis by Str. chrysomallus var. macrotetrolidi.
    • To analyze the relationship between phosphate levels, intracellular ATP content, hemin accumulation, and nonactin production.
    • To understand the metabolic state of the actinomycete during antibiotic synthesis.

    Main Methods:

    • Cultivation of Str. chrysomallus var. macrotetrolidi with varying potassium orthophosphate concentrations.

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  • Measurement of mycelial growth and nonactin antibiotic content.
  • Quantification of intracellular adenosine triphosphate (ATP) and hemin levels.
  • Analysis of protein content in the mycelium.

    • Main Results:

    • Potassium orthophosphate addition directly correlated with intracellular ATP levels in the mycelium.
    • Intracellular ATP content exhibited a biphasic pattern related to mycelial age.
    • Hemin levels were influenced by mycelial age and phosphate availability.
    • Nonactin biosynthesis was inversely correlated with intracellular ATP, protein, and hemin concentrations.
    • Peak antibiotic production occurred when ATP levels were decreasing.

    Conclusions:

    • Phosphate availability significantly modulates ATP metabolism and nonactin biosynthesis in Str. chrysomallus var. macrotetrolidi.
    • A low intracellular ATP state, coupled with reduced protein and hemin, favors intensive nonactin production.
    • Understanding these metabolic dynamics can optimize conditions for industrial-scale antibiotic manufacturing.