Pseudomonas paralcaligenes sp. nov., isolated from a hospitalized patient

A Gram-stain-negative, aerobic, rod-shaped, non-endospore-forming bacterium, designated as strain MRCP1333^T, was isolated from a faecal sample from a hospital patient in Japan. MRCP1333^T grew at temperatures of 15-40 °C (optimum 25-35 °C), with 1.0-3.0 % (w/v, 171-513 mM) NaCl [optimum 1-2 % (w/v), 171-342 mM], and at pH 6.0-9.5 (optimum pH 7.0-8.0). The results of phylogenetic analysis based on the sequences of the 16S rRNA gene and the 53 genes encoding the bacterial ribosome protein subunits indicated that MRCP1333^T represented a member of the Pseudomonas aeruginosa group, most closely related to Pseudomonas alcaligenes. Whole-genome comparisons, using average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity, confirmed that MRCP1333^T represented a distinct species in the P. aeruginosa group. Phenotypic characterization tests demonstrated utilization by this strain of citrate, glycerol, and d-malic acid, the ability to reduce nitrite to nitrogen and the ability of this strain to grow in the presence of minocycline and tetrazolium blue, distinguishing this strain from P. alcaligenes and other closely related species of the P. aeruginosa group. The major fatty acids of MRCP1333^T were summed feature 8 (C_18 : 1ω7c/C_18 : 1ω6c; 38.4 %), summed feature 3 (C_16 : 1ω7c/C_16 : 1ω6c; 21.1 %) and C_16 : 0 (20.6 %). The DNA G+C content of MRCP1333^T was 66.5 mol%. Genetic and phenotypic evidence indicated that MRCP1333^T should be classified as representing a novel species, for which the name Pseudomonas paralcaligenes sp. nov. is proposed. The type strain is MRCP1333^T (=LMG 32254^T,=JCM 34250^T).