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Anti-cancer activity of ultra-short single-stranded polydeoxyribonucleotides

Alexander S Vedenkin1, Sergey V Stovbun2, Alexander A Bukhvostov3

  • 1N.N. Semenov Federal Research Center for Chemical Physics RAS, Moscow, Russia. a.s.vedenkin@gmail.com.

Investigational New Drugs
|February 7, 2023
PubMed

Insights

Ultra-short single-stranded deoxyribose polynucleotides (ssDNA) show promise as novel anti-cancer drugs. These compounds effectively inhibit DNA polymerase, suppressing cancer cell proliferation and tumor growth in preclinical studies.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Cancer cells exhibit increased proliferation rates, presenting a therapeutic target.
  • DNA replication and repair are critical for cell survival and proliferation.
  • Inhibiting DNA replication machinery can lead to cancer cell death.

Purpose of the Study:

  • To investigate the anti-tumor potential of ultra-short single-stranded deoxyribose polynucleotides (ssDNA).
  • To evaluate the efficacy of ssDNA in inhibiting DNA polymerase activity.
  • To assess the therapeutic effects of ssDNA on cancer development and tumor growth.

Main Methods:

  • In vitro studies using cancer cell cultures.
  • In vivo studies using mouse cancer models.
  • Assay of DNA polymerase catalytic activity inhibition by ssDNA.

Main Results:

  • ssDNA effectively inhibits the catalytic activity of DNA polymerase.
  • ssDNA demonstrated significant suppression of cancer development in vitro.
  • ssDNA significantly suppressed tumor growth in vivo mouse models.

Conclusions:

  • Ultra-short single-stranded deoxyribose polynucleotides are effective inhibitors of DNA polymerase.
  • ssDNA exhibits significant anti-tumor activity, warranting further investigation as a novel cancer therapeutic agent.
Keywords:
CancerDNA repairSingle-stranded DNAcytostatics

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