Stem Cell-Based Modeling Protocol for Parkinson's Disease

Parkinson's disease is a progressive neurodegenerative disorder, which is mainly characterized by unintended or uncontrollable body movements. Pathophysiologically, disturbances in the neurotransmission system of the brain like dopaminergic system and synaptic dysfunction are classified as top-rated causes of the onset of Parkinson's disease, which symptoms can be different according to the involvement of neurotransmission system type and the effect of the disease on the motor and non-motor systems. Although some pharmacological and non-pharmacological approaches have been applied to control and slow down the progression of the disease, a definitive cure has not yet been discovered. One of the factors involved in this issue is the lack of appropriate laboratory models to investigate the pathological mechanisms involved in the disease as well as various aspects of candidate drugs, which ultimately leads to the failure of drug discovery and development pipelines. To deal with these challenges, the application of stem cells, especially cellular reprogramming of somatic cells to human pluripotent stem cells, also known as induced pluripotent stem cells, has been able to promise a new chapter in the modeling of Parkinson's disease. Induced pluripotent stem cells have the stemness capability; therefore, they can differentiate into any type of cell such as nerve cells. Also, since these cells are obtained from the reprogramming of somatic cells in the patient's body, they maintain the patient's genetic content, which can play an important role in increasing the quality of disease modeling and the validity of the results of laboratory studies. Therefore, the procedure for modeling induced pluripotent stem cells for Parkinson's disease is explained in this chapter.