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Updated: Aug 11, 2025

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

Stem Cell-Derived Microfluidic Amniotic Sac Embryoid (μPASE).

¹Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)

|February 7, 2023

Summary

Researchers developed a microfluidic amniotic sac embryoid (μPASE) model using human pluripotent stem cells. This controllable system mimics early human embryonic development and germ cell specification.

Keywords:

Amnion Embryonic development Human embryoid Human pluripotent stem cell Microfluidics Primitive streak Primordial germ cell

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Last Updated: Aug 11, 2025

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Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

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Published on: February 28, 2017

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Published on: September 6, 2014

Area of Science:

Developmental Biology
Stem Cell Biology
Bioengineering

Background:

Early human embryonic development post-implantation is complex and challenging to study.
Human pluripotent stem cells (hPSCs) offer a model system for recapitulating developmental processes.
Existing models may lack the control and scalability needed for detailed investigation.

Purpose of the Study:

To develop and characterize a microfluidic amniotic sac embryoid (μPASE) model.
To establish a controllable, reproducible, and scalable system for studying early human embryogenesis.
To provide detailed protocols for μPASE generation and characterization.

Main Methods:

Utilizing a microfluidic device to control hPSC cluster formation and morphogen exposure.
Culturing hPSC-derived embryoids in a controlled microenvironment.
Employing immunofluorescence staining and cell retrieval for characterization.

Main Results:

The μPASE model successfully recapitulates key features of early post-implantation human embryonic development.
Observed developmental landmarks include epiblast lumenogenesis and amniotic cavity formation.
Specified primordial germ cells and gastrulating (mesendoderm) cells were identified within the μPASEs.

Conclusions:

The μPASE system provides a robust and scalable platform for studying human embryonic development.
This model facilitates the investigation of early human embryogenesis, including germ cell and mesendoderm specification.
Detailed protocols are provided for the reproducible generation and analysis of μPASEs.