Surface Tailoring of 3D Scaffolds to Promote Osteogenic Differentiation

Customized bone scaffolds with osteogenic activities are desired for the regenerative repair of large-scale or irregularly shaped bone defects. This study developed a facile method to create osteogenic surfaces on three-dimensional (3D) printed scaffolds through coating-induced mineralization. The coating was synthesized using chemical vapor deposition of a polyelectrolyte containing oppositely charged groups. The opposite charges on the 3D scaffold played a crucial role in promoting the formation of nanoapatites without agglomeration, resulting in the retention of micro- and nanoscale pore openings needed for preosteoblasts to proliferate, differentiate, and migrate. The nanoapatite scaffold exhibited significant enhancement in osteoinductivity with a 107% increase in alkaline phosphatase expression and a 163% increase in osteocalcin activity compared to the pristine scaffold. The nanoapatite scaffold provided cues for preosteoblasts to grow along aligned features and migrate collectively. The findings of this study demonstrate the synergistic effect of oppositely charged polyelectrolytes and mineralized nanoapatites on promoting osteogenic activities on scaffold surfaces.