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Reflectional quantitative differential phase microscopy using polarized wavefront phase modulation.

Ying Ma¹, Taiqiang Dai², Lan Yu¹

¹School of Physics, Xidian University, Xi'an, China.

Journal of Biophotonics

|February 8, 2023

Summary

This summary is machine-generated.

A new reflectional quantitative differential phase microscopy (RQDPM) offers label-free imaging for opaque and biological samples. This technique provides high resolution and can switch between reflection and transmission modes for diverse applications.

Keywords:

full-aperture illumination label free partially coherent polarized wavefront modulation quantitative differential phase reflectional imaging

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Area of Science:

Optics and Photonics
Biomedical Imaging
Materials Science

Background:

Quantitative phase microscopy (QPM) is a crucial label-free technique for imaging.
Existing QPM methods have limitations in imaging opaque surfaces and turbid biological specimens.

Purpose of the Study:

To introduce a novel reflectional quantitative differential phase microscopy (RQDPM) technique.
To demonstrate RQDPM's applicability to opaque surfaces and biological samples.

Main Methods:

Developed RQDPM using polarized wavefront phase modulation and partially coherent full-aperture illumination.
Implemented a system that can switch between reflection and transmission modes.
Leveraged principles similar to differential interference contrast microscopy for axial resolution.

Main Results:

Achieved high spatial resolution and spatio-temporal phase sensitivity.
Successfully performed reflectional phase imaging on semiconductor wafers, thick biological tissues, red blood cells, and Hela cells.
Dynamically monitored microsphere flow in microfluidic channels using transmission mode.

Conclusions:

RQDPM is a versatile, label-free imaging tool for both opaque and transparent specimens.
The technique offers high resolution and adaptable imaging modes.
RQDPM has potential applications in materials inspection and advanced biological studies.