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Updated: Aug 11, 2025

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Muscle Imbalances: Testing and Training Functional Eccentric Hamstring Strength in Athletic Populations
Published on: May 1, 2018
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Activity distribution among the hamstring muscles during high-speed running: A descriptive multichannel surface EMG
Jozef J M Suskens1,2,3, Johannes L Tol2,3,4, Gino M M J Kerkhoffs1,2,3
1Department of Orthopedic Surgery and Sports Medicine, Amsterdam UMC Location University of Amsterdam, Amsterdam, The Netherlands.
Scandinavian Journal of Medicine & Science in Sports
|February 8, 2023
Summary
Hamstring muscles show varied activity during high-speed running, with semimembranosus most active in the late-swing phase. Peak activity occurred at consistent joint angles across all hamstring muscles.
Area of Science:
- Biomechanics
- Human Movement Science
- Sports Medicine
Background:
- Understanding hamstring muscle activation patterns is crucial for optimizing athletic performance and preventing injuries.
- High-speed running involves complex neuromuscular coordination of the hamstring group.
Purpose of the Study:
- To analyze the distribution of muscle activity within and between hamstring muscles during high-speed running.
- To compare the relative contribution of each hamstring muscle and identify joint angles at peak activity.
Main Methods:
- Multichannel electromyography (EMG) was used to measure hamstring muscle activity in male basketball players during treadmill running.
- Activity was recorded at 15 specific locations across the biceps femoris long head, semitendinosus, and semimembranosus.
- Muscle activity was analyzed for each location throughout the stride cycle.
Main Results:
- Heterogeneous muscle activity was observed across all hamstring muscles during different phases of the running stride.
- Semimembranosus exhibited significantly higher activity and relative contribution than semitendinosus during the late-swing phase.
- Peak muscle activity occurred at similar hip and knee joint angles, irrespective of the specific hamstring muscle or location.
Conclusions:
- Hamstring muscles are most active during the late-swing phase of high-speed running.
- Semimembranosus demonstrates the highest activity, while semitendinosus shows the least activity in this phase.
- Proximal regions of the biceps femoris long head were more active in the late-swing phase compared to other regions.

