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Updated: Aug 11, 2025

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
Published on: March 25, 2009
Observing hydrogen sulfide in the endoplasmic reticulum of cancer cells and zebrafish by using an activity-based
Yves S Kafuti1,2, Shuang Zeng2, Xiaosheng Liu2
1State Key Laboratory of Fine Chemicals, Frontiers Science Center for Smart Materials Oriented Chemical Engineering, Dalian University of Technology, 2 Linggong Road, Dalian, 116024, China. lihd@dlut.edu.cn.
Abstract:
A crucial endogenous signaling chemical, hydrogen sulfide, is involved in many physiological actions. In this work, we created the fluorescent probe ER-Nap-NBD using a naphthalimide fluorophore as the signal reporter, a 7-nitro-1,2,3-benzoxadiazole amine as the responsive moiety, and a sulfonamide part for endoplasmic reticulum targeting. ER-Nap-NBD could be detected the H2S levels in solution and in living systems (cells and zebrafish).

