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Multiple effects of the PHO91 gene knockout in Ogataea parapolymorpha
Vasilina Farofonova1,2, Nadeshda Andreeva1, Ekaterina Kulakovskaya1
1Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino, Russian Federation.
Folia Microbiologica
|February 8, 2023
Summary
Knocking out the PHO91 gene in yeast impacts cellular processes, affecting stress resistance and methanol metabolism. This study reveals pleiotropic effects of PHO91 deletion in Ogataea parapolymorpha.
Area of Science:
- Cellular and Molecular Biology
- Microbiology
- Biochemistry
Background:
- Pho91 is a vacuolar phosphate transporter crucial for phosphate export in yeast.
- Understanding its function is key to cellular phosphate homeostasis.
Purpose of the Study:
- To investigate the pleiotropic effects of PHO91 gene knockout in Ogataea parapolymorpha.
- To analyze the impact on polyphosphate levels, stress resistance, and methanol metabolism.
Main Methods:
- Gene knockout of PHO91 in Ogataea parapolymorpha.
- Measurement of acid-soluble and acid-insoluble inorganic polyphosphate (polyP).
- Assessment of resistance to cadmium, manganese, and peroxide stresses.
- Analysis of methanol consumption and methanol oxidase activity.
Main Results:
- ∆pho91 cells showed slightly higher polyphosphate content compared to wild-type on glucose.
- pho91-Δ mutations decreased cadmium resistance but increased resistance to manganese and peroxide.
- Mutant O. parapolymorpha cells lost the ability to consume methanol due to reduced methanol oxidase activity.
Conclusions:
- PHO91 knockout leads to significant alterations in yeast physiology, including stress response and metabolic capabilities.
- These effects may stem from impaired vacuolar phosphate mobilization and altered signaling pathways involving phosphates and polyphosphates.
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