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Updated: Aug 11, 2025

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
Published on: September 21, 2018
Initial ciliary assembly in Chlamydomonas requires Arp2/3 complex-dependent endocytosis
Brae M Bigge1, Nicholas E Rosenthal1, Prachee Avasthi1
1Biochemistry and Cell Biology Department, Geisel School of Medicine at Dartmouth College, Hanover, NH 03755; Anatomy and Cell Biology Department, University of Kansas Medical Center, Kansas City, KS 66103.
Abstract:
Ciliary assembly, trafficking, and regulation are dependent on microtubules, but the mechanisms of ciliary assembly also require the actin cytoskeleton. Here, we dissect subcellular roles of actin in ciliogenesis by focusing on actin networks nucleated by the Arp2/3 complex in the powerful ciliary model, Chlamydomonas. We find that the Arp2/3 complex is required for the initial stages of ciliary assembly when protein and membrane are in high demand but cannot yet be supplied from the Golgi complex. We provide evidence for Arp2/3 complex-dependent endocytosis of ciliary proteins, an increase in endocytic activity upon induction of ciliary growth, and relocalization of plasma membrane proteins to newly formed cilia.
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